$11 | AQs in BTN | PKO

This is a hand you can put in your 3bet range, but playing this deep I wouldn't mind the flat in position.
As played you beat only a bluff on the river and read less I would fold middle pair.

Cheers,
SDK1987

First I was thinking that I made a mistake by not 3-betting pre-flop, on the flop I don't believe I can fold to a 1/4 pot bet, same on the turn when I picked up some more outs, (and his bet sizing is screaming "pay me" ). But the big mistake is me calling the river...I was thinking that this is a good spot to bluff but then I remember I am playing micro stake and players do not bluff that often...and still made the call

Nevertheless here is the full hand:

PokerStars - 125/250 Ante 30 NL (8 max) - Holdem - 8 players
Hand converted by Holdem Manager 3

UTG: 22,947 (91.8 bb)
UTG+1: 58,786 (235.1 bb)
MP: 41,922 (167.7 bb)
MP+1: 37,275 (149.1 bb)
CO: 18,860 (75.4 bb)
Hero (BTN): 31,852 (127.4 bb)
SB: 24,690 (98.8 bb)
BB: 24,850 (99.4 bb)

8 players post ante of 30, SB posts 125, BB posts 250

Pre Flop: (pot: 615) Hero has Q♦: A♦:
3 folds, MP+1 raises to 625, fold, Hero calls 625, fold, BB calls 375

Flop: (2,240, 3 players) 5♠: K♠: Q♣:
BB checks, MP+1 bets 672, Hero calls 672, fold

Turn: (3,584, 2 players) T♥:
MP+1 bets 1,075, Hero calls 1,075

River: (5,734, 2 players) 5♥:
MP+1 bets 4,301, Hero calls 4,301

Results: 14,336 pot (0 rake)
Final Board: 5♠: K♠: Q♣: T♥: 5♥:

MP+1 shows Q♥: Q♠:: (Full House, Queens full of Fives)
(Pre 66%, Flop 98%, Turn 91%)

Hero mucks Q♦: A♦:: (Two Pair, Queens and Fives)
(Pre 34%, Flop 2%, Turn 9%)

MP+1 wins 14,336

Even on the higher stakes this will be a tough call to make on the river I think.
Only missed flush draw could 3barrel bluff I think.